We are seeking a highly motivated candidate with a background in Stem Cell Biology, Cell Biology, Molecular Genetics, and/or Genetics for a post-doctoral position within an independent research group in the Center for Genes, Environment, and Health. The research in our laboratory focuses on translational genetic and genomic studies of complex lung diseases. This position will be focused on determining the molecular function of genetic disease variants in airway epithelial cells using primary cell culture systems.
The successful candidate will use cutting edge molecular tools including CRISPR's to edit the genome of primary airway and iPS cells to study the function of genetic disease variants. This candidate will also lead studies investigating the molecular effects of environmental exposures on airway epithelial cells and the differentiation of pluripotent cells into airway progenitors. The successful applicant will be responsible for performing experiments in the laboratory, which will include tissue culture, cloning, cell exposures and infections, flow cytometry, transfections, real-time PCR, Nex-gen sequencing, fluorescent microscopy, and various cellular assays. The successful applicant will be expected to learn and develop techniques as required by the research project. The successful applicant will be expected to carry out data analysis from experiments conducted and participate in manuscript preparation and writing.
The ideal candidate will display:
- Ph.D. in Stem Cell Biology, Cell Biology, Molecular Genetics, Genetics, Immunology, or a related field.
- Excellent written and verbal communication
- Effective judgment and problem solving with little supervision
- Highly motivated and committed to biomedical research
- Multi-tasking and a willingness to learn new technologies
- Cell culturing skills
- Molecular biology skills
- Primary airway epithelial culture
- iPSC derivation and differentiation
- Work with Genome-editing technologies
- Gene expression work including Q-PCR or Nex-gen sequencing
- Microscopy skills
- Flow Cytometry
1. Performs, on a very independent basis, all phases of experimental genetics/genomics investigations, to include being responsible to the Principal Investigator in the planning, developing, operating, recording, collaborating and reporting of major genetics/genomics projects.
2. Collaborates with Principal Investigators and senior researchers in the progress of major research projects.
3. Provides guidance to laboratory staff in one or more laboratory areas, including technicians, support and auxiliary staff. Performs as the technical advisor on complex analyses of gene and genetic variant function through the performance and use gene-editing technologies and 2D, 3D, organoid cell culture models, and other sophisticated techniques.
4. Serves as primary contact with researcher staff for technical services on major genetic/genomic techniques, recording devices and statistical procedures relative to the specifically assigned field.
5. Reviews quality control of new or modified laboratory techniques which affect any part of major projects.
6. Performs routine and non-routine tests leading to the successful completion of developed experiments. Knows and follows age-specific guidelines.
7. Performs as a back-up to staff absences and vacancies, as necessary.
8. Prepares scientific manuscripts related to the genetic/genomic analyses conducted.
9. Performs all other duties as assigned.
1. Performs, as directed, safety compliance and uses Personal Protective Equipment (PPE), as needed.
2. Participates in Quality Assessment (QA) and Quality Improvement (QI) programs, as directed.
3. Ensures compliance with The Joint Commission and all other Federal, State and Regulatory Agencies.
4. Responds promptly and sincerely to customer's needs, requests and concerns via all communication forms using easily understood language and refraining from using inappropriate language and non-verbal gestures.
5. Maintains positive working relationships as a team player through problem solving issues, speaking positively about others, listening attentively and observing the Patient Bill of Rights and Confidentiality.
6. Increases customer service knowledge, skill and ability by participating in department and institution-wide specific programs.
7. Incorporates National Jewish's identity (Science Transforming Life®) into daily functions. Speaks positively about the institution, provides customers with prompt service, maintains a clean and safe working environment, dresses appropriately based upon National Jewish safety standards, and departmental policies and wears an ID badge visibly.
Knowledge and Skills
Extensive knowledge of standard research practices and methods in the conduct of biomedical research, but specifically in the conduct of gene-editing and complex 2D, 3D, and organoid tissue culture systems. Ability to design and plan complex genetic and molecular biology experiments. Ability to determine the approach to a research problem which will give the most significant results and to develop, lay out and plan the actual experiments to be performed, and ability to devise new methods and techniques as needed in experimentation. Ability to plan, organize and direct work of supporting personnel. Ability to prepare and write reports on research projects. Ability to establish and maintain effective working relationships with associates, other research personnel and the general public.
M. D.; or, Ph. D. in genetics, genomics, biology, chemistry or other basic sciences required.
Certification and Licensure
Experience acquired in earning a Ph. D. in a specified basic science field.
Position is in a laboratory. Frequent requirements may include: manual dexterity; normal color vision; long periods of standing and sitting, and frequent periods of bending, crouching or stooping; work with radioactive materials, toxic chemicals, biomedical wastes and research animals; potential exposure to infectious disease; work around electrical/explosive hazards; work with laboratory equipment/machinery; and lifting objects weighing 10 to 50 pounds; and extensive repetitive motion of the fingers, arm and wrist. Occasional requirements may include: sitting while completing paperwork, kneeling, and squatting. Incumbent may be scheduled to work an alternate schedule to accommodate essential business needs routine travel by foot or automobile to alternate work/meeting locations, possibly during inclement weather.
Inside environmental conditions may include: working in laboratories with odors, chemicals and animals; extreme temperatures; electrical; working around hazardous and radioactive substances; working under dirty and dusty conditions. Personal Protective Equipment (PPE) will be provided to each employee when needed as determined by policy. Utilization of PPE is mandatory. Estimated 1700 employees in the worksite.